Last updated 26th May 2025

OECD 208 Test for Emergence and Growth of Terrestrial Seedlings

The OECD 208 biodegradation test assesses the effect of the test substance on seedling emergence and their early growth, with exposure occurring in or on the soil. The test uses different soil batches where the test substance is applied, which are then assessed for endpoint measurements (including emergence of seedlings, biomass, shoot height, and visible adverse effects).

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    OECD 208 Introduction

    OECD 208 analyzes the effects of chemicals on the germination of seedlings and the early stages of growth of terrestrial plants. It is used to assess the phytotoxicity of a variety of materials, including pesticides, industrial chemicals, and soil contaminants. This test helps identify if a chemical could pose a risk to plants in either agricultural or natural settings.

    OECD 208 Test Method

    Test procedure The terrestrial plants are placed in the soil that has been treated with the test substance.

    OECD 208 Equipment and Sample Preparation

    Specimen DetailsFor the test sample is soluble in water, it is dissolved in water and then mixed with soil. For the test sample is of low solubility, it is dissolved in suitable volatile solvent, such as acetone or ethanol, then mixed with soil. For the insoluble test sample , the chemicals are directly mixed with dry soil. 
    Specimen PreparationThe most common plants used in this experiment are carrots, sunflowers, lettuce, cucumber, soybean, onion, rice, wheat, corn, and other typical plants. They are planted in natural soils, including sandy loam, loamy sand, sandy clay loam, or in commercially available potting soil equally not exceeding 1.5% organic carbon. Seeds are positioned in contact with soil that has been treated with the test substance and observed for effects typically 14 to 21 days after 50% of the seedlings in the control group have emerged.

    OECD 208 Results and Interpretation

    At the end of this test, we assess seedling emergence, biomass measurements, shoot height, and any visible harmful effects on various parts of the plant. Several criteria must be met for the test to be considered valid, including:

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    1. A minimum seedling emergence of 70% for the blank control  

    2.No visible phototoxic effects on the control seedlings  

    3. At least 90% mean survival rate of the emerged control seedlings throughout the study     duration  

    Tests Related to OECD 208

    ASTM D6400 test to evaluate the effects of composting products of plastic materials on seedlings with OECD 208.

    FAQ's

    Where can I get the oecd 208 tested?

    You can share your oecd 208 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 208 test to various testing techniques.

    How much do I need to pay for the oecd 208 test?

    Please contact us for a detailed quote for your oecd 208 testing needs. Cost incurred to carry out different oecd 208 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

    How many samples are required for oecd 208?

    The required number of samples or specimens should comply with the procedure given in the oecd 208 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

    How much discount can I get on the oecd 208 test?

    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

    How many days will it take to complete the oecd 208 test?

    The turnaround time for oecd 208 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the oecd 208 tested?

    You can share your oecd 208 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 208 test to various testing techniques.

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