ASTM D5988 Standard Test Method for Determining Aerobic Biodegradation in Soil of Plastic Materials or Residual Plastic Materials After Composting

    What is ASTM D5988?

    ASTM D5988 is a standard test method for determining the aerobic biodegradation of plastic materials or plastic residuals after soil composting. The biodegradation method is based on the change from carbon in the material to carbon dioxide (CO₂) within a controlled aerobic soil environment.  Technicians set up the test, add the material to a suitable soil matrix, and include controls. They then measure the amount of carbon dioxide released during the test period. Analysts use the released carbon dioxide to calculate the biodegradation rate. ASTM D5988 (Standard Test Method for Determining Aerobic Biodegradation in Soil of Plastic Materials or Residual Plastic Materials After Composting)  is useful for evaluating how plastic materials perform in soil and for indicating how biodegradable the material is under controlled conditions.

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    Get Certified ASTM D5988 Biodegradation Testing

    ASTM D5988 testing helps manufacturers, material developers, researchers, and environmental professionals assess plastic materials for aerobic biodegradation performance in soil. Technicians carefully prepare the soil, test material, controls, and test vessels before beginning the exposure. Analysts monitor carbon dioxide production throughout the test and evaluate the accumulated carbon dioxide relative to the carbon content of the test material. The results can support material development, environmental assessment, and comparison of biodegradable plastic materials.

    What is the scope of ASTM D5988?

    ASTM D5988 specifies the methods for determining aerobic biodegradation in soil of plastic materials or the remaining plastic materials after composting. This approach involves quantifying the amount of CO₂ generated by the test material when it is stored in soil under controlled conditions. ASTM D5988 is primarily used to assess the level of aerobic biodegradability of plastic materials in soil based on the amount of CO₂ generated during the test compared to the amount of CO₂ that would be generated from the plastic. ASTM D5988 covers: 

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    • Material Type: Plastic materials and residual plastic materials remaining after composting.
    • Purpose: Determination of aerobic biodegradation in soil.
    • Property Measured: Carbon dioxide evolution resulting from biodegradation.
    • Method: Technicians expose the test material to soil under controlled aerobic conditions and monitor CO₂ evolution over time.
    • Result: Biodegradation is expressed as a percentage (%) of theoretical carbon dioxide production.
    • Test Limitations: Results depend on soil characteristics, moisture, temperature, test duration, material properties, and microbial activity.
    • Environmental Conditions: The soil environment is maintained under controlled aerobic conditions suitable for microbial biodegradation.
    • Applications: Biodegradable plastic evaluation, material development, environmental assessment, compost-residue evaluation, and product comparison.

    What Are the Uses of ASTM D5988 Testing?

    ASTM D5988 testing can assess whether plastic materials are biodegradable in soil. The process quantifies the amount of CO₂ emitted as microbes decompose the plastic. Allowing the number to indicate the extent of biodegradation.  This provides a number that shows how much biodegradation has occurred. Manufacturers and researchers use these results to compare plastic materials. They also use the data to see how well a material biodegrades over time or after changes are made to it. The test helps to:

    • Biodegradation Evaluation: Measures the amount of plastic biodegraded in soil under aerobic conditions.
    • Biodegradability comparison: Compares biodegradability behavior between plastic formulations.
    • Product Development: Supports development and optimization of biodegradable plastics.
    • Environmental Assessment: Gives info on plastic behavior in soil.
    • Compost Residue Evaluation: Test of plastic residues after composting.
    • Quality Evaluation: Facilitates evaluation of the biodegradation performance in relation to the applicable requirements.
    • Research: Provides data for studies of plastic degradation and soil biodegradation.

    Which Materials Can Be Tested According to ASTM D5988?

    ASTM D5988 applies to plastic materials that can be evaluated under controlled aerobic soil conditions. The method also addresses residual plastic materials obtained after composting when their subsequent biodegradation in soil needs to be determined. Technicians prepare representative test materials and expose them to suitable soil containing active microorganisms. Analysts compare the resulting CO₂ evolution with appropriate controls and theoretical carbon dioxide production. The method applies to-

    • Material Type: Plastic materials suitable for aerobic soil biodegradation testing.
    • Residual Materials: Plastic residues remaining after composting.
    • Biodegradable Plastics: Plastic formulations developed to undergo biological degradation.
    • Production Materials: Representative plastic samples collected from manufacturing processes.
    • Development Materials: Experimental or modified plastic formulations under development.

    Why is ASTM D5988 Testing Important?

    Plastic materials can undergo different changes when they get in soil, and the mere fact that they are not visible doesn’t mean that they have biodegraded. ASTM D5988 is a quantitative method based on the measurement of carbon dioxide produced during microorganisms’ metabolism of the test material. The resulting biodegradation data aids manufacturers, researchers, and environmental professionals to better understand the behavior of plastic materials in controlled aerobic soil conditions. It can also be used to compare formulations and develop formulations that degrade in soil.

    ASTM D5988 Equipment and Sample Preparation Guide

    Technicians prepare representative plastic specimens, suitable soil, test vessels, and carbon dioxide measurement equipment before starting the test. They prepare control systems alongside the test material so analysts can distinguish biodegradation-related CO₂ production from background soil respiration. Specimen dimensions or particle size should follow the applicable procedure and provide sufficient surface area for representative exposure to the soil.

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    Test Soil and Sample PreparationTechnicians provide an active aerobic environment and prepare representative plastic material for exposure.
    Test Vessels and Incubation SystemTechnicians maintain the soil and plastic specimens under controlled test conditions.
    Specimen DimensionsASTM D5988 test specimens can be introduced into the soil matrix directly or after composting. In composting cases, a homogeneous, representative sample of residual plastics is used to reflect real-world conditions and enable a comprehensive evaluation of aerobic biodegradation post-composting.
    CO₂ Collection/Measurement EquipmentTechnicians capture and measure carbon dioxide generated during biodegradation.
    Controls and Analytical EquipmentThe technician establishes background respiration and supports accurate biodegradation calculations.

    ASTM D5988 Testing Procedures and Requirements

    Technicians first prepare the soil and representative plastic test material and establish the required control systems. They place the test material into the soil and maintain the specified aerobic conditions throughout the exposure period. The technician periodically measures carbon dioxide evolution from the test system and records the accumulated CO₂ production. Analysts correct the measurements using the appropriate controls and calculate the percentage of theoretical carbon dioxide production attributable to the test material. The testing process includes the following steps.

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    Soil and Specimen PreparationTechnicians establish representative soil and plastic test systems.
    Control PreparationTechnicians measure background soil respiration and provide a basis for data correction.
    Aerobic Soil ExposureThe analyst exposes the plastic material to controlled microbial soil conditions.
    CO₂ MonitoringTechnicians measure carbon dioxide generated during biodegradation.
    Data Correction and CalculationTechnicians account for CO₂ production in the control and determine the biodegradation percentage.
    Result EvaluationThe analyst assesses the extent of biodegradation over the test period.

    ASTM D5988 Testing Process and Data Collection

    Technicians first prepare soil and plastic test material, controls, and test vessels. They provide the above-mentioned aerobic environment and add the plastic to the soil. Technicians measure the amount of carbon dioxide produced during incubation at the appropriate times. The CO₂ data is collected and aggregated by analysts, background CO₂ is subtracted, and the percentage of theoretical carbon dioxide production is calculated. The resulting biodegradation profile gives information on the level and rate of biodegradation of plastic in the given soil conditions.

    ASTM D5988 process showing plastic sample preparation, soil incubation, controlled aerobic exposure, carbon dioxide collection, CO₂ measurement, data analysis, and biodegradation calculation.
    ASTM D5988 Aerobic Biodegradation Testing of Plastic Materials in Soil

    Common Challenges and Troubleshooting

    Variations in soil moisture, temperature, microbial activity, aeration, specimen preparation, and carbon dioxide measurement can influence ASTM D5988 results. Technical staff should be responsible for maintaining uniformity of soil conditions, taking representative samples, not contaminating the soil samples, and testing the CO₂ measuring system. Analysts should check soil activity, soil moisture, soil temperature, control performance, specimen preparation, measurement instrumentation, and test conditions if CO₂ production is found to be different from expected.

    Analysis Results and Interpretation

    The report summarizes the aerobic biodegradation of the tested plastic material in soil. It presents the calculated biodegradation result together with relevant sample identification, specimen information, carbon dioxide measurements, test duration, incubation conditions, and other required test information.

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    • ASTM D5988 produces biodegradation-specific results based on carbon dioxide evolution rather than a single general material-property value.
    • Analysts commonly report biodegradation as a percentage of theoretical carbon dioxide production (%), while carbon dioxide measurements may be reported in mg CO₂ or other applicable SI units. Relevant specimen mass measurements may be reported in g.
    • The report contains the following information: identification of the plastic material, specimen data, soil conditions, incubation conditions, test duration, carbon dioxide measurements, control results, and calculated percentage of biodegradation.
    • By ensuring that specimen preparation, soil preparation, incubation, carbon dioxide measurement, handling of controls, and equipment calibration are well-documented, engineers can achieve reliable, comparable results.

    Link to ASTM D5988 

    FAQ

    What does ASTM D5988 measure?
    ASTM D5988 is used to measure the aerobic biodegradability of plastic materials or remaining plastic after composting in soil.
    The technique tracks the carbon dioxide generated from the soil microbes’ aerobic decomposition of the plastic substrate.
    The primary result is the percentage of theoretical carbon dioxide production (expressed as % biodegradation).
    The method evaluates suitable plastic materials and residual plastic materials remaining after composting.
    Carbon dioxide production provides a quantitative indicator of microbial conversion of carbon from the plastic material during aerobic biodegradation.

    Updated on September 7, 2026

    Malhar Khole
    About Author
    Malhar Khole is a Material Testing Associate at MaTestLab Inc. and holds a postgraduate degree in Material Science and Technology from IIT (BHU), Varanasi.
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