Last updated 21st February 2025

ASTM E640 Standard Test Method for Preservatives in Water-Containing Cosmetics

ASTM E640 is the standard test for determining the efficacy of preservatives in water-soluble cosmetics to prevent microbial growth. Cosmetics are widely used in the beauty industry these days, and ensuring their quality is essential. This method assesses the effectiveness of preservatives in model formulations to ascertain that preservatives satisfy performance benchmarks acceptable for cosmetic products.

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    ASTM E640 Introduction

    ASTM E640 is the standard important for assessing the effectiveness of preservatives for water-soluble cosmetics to protect against microbial contamination. This method evaluates model formulations of preservatives to ensure that the preservatives meet performance criteria appropriate for the cosmetics industry. 

    ASTM E640  Test Method

    Culture inoculation and incubationThe bacterial cultures are streaked on the right kind of slants using a clean inoculum.  The evolution from each culture is put into tubes of permanent cycles. The yeast cultures are then harvested, and the suspension is adjusted using sterile saline. To minimize the time needed to perform the assay, pre-streaking of the challenge inoculum is done by creating serial dilutions. Lethean agar is then employed to make a set of replicas of the plates. Bacteria and yeast are sometimes grown.
    Testing for preservation abilityThe test is prepared by weighing twenty grams of aliquots of substance into suitable glass jars. The diluted test samples are spread on bacteria and fungi recovery agars. Gram staining is a method of distinguishing bacteria. They are examined periodically for infected samples. It is tested at the minimum interval of inoculated samples.

    ASTM E640 Equipment and Sample Preparation

    Specimen DetailThe test contains formulations that the submitter considers suitable for demonstrating preservative activity. These formulas also contain non-preserved (control) samples.
    Specimen SizeMicroorganisms are used to be cultured and tested for activity.

    ASTM E640 Results and Interpretation 

    The validity of the preservative efficacy test is determined by the counts of the initial inoculum in ASTM E640. This means that the microbial challenge starts with a predetermined number of organisms.

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    Tests Related to ASTM E640 

    ASTM E640 is related to tests, including ASTM E1054, for inactivators of antibacterial compounds.

    Use of ASTM E640 Testing in Various Industries

    This testing is widely applied in different industries, including cosmetic, pharmaceutical, and personal care. Its primary use is to evaluate preservative efficacy so that water-based cosmetic products remain microorganisms-free and safe for the consumer. ASTM E640 testing in the cosmetics manufacturing industry ensures that preservative solutions provide adequate control of microorganisms necessary to extend the shelf life of cosmetic products and protect the consumer.

    Materials Commonly Tested with ASTM E640

    Substances most often examined for this test essentially encompass water-soluble cosmetic products that may need stabilization against microbial development. This covers a broad spectrum of products, including creams, lotions, shampoos, conditioners, and most personal products created from water or with water content. The test also assesses the efficiency of preservatives used in these goods so that they can effectively prevent bacterial, yeast, and mold growth throughout the useful life of the product.

    Safety and Best Practices in ASTM E640  Testing

    Some safety and best practices should be considered when using any explosive when conducting this testing. Such measures include the use of proper individual personal protective apparel (PPE), maintaining clean, putrid-less surfaces and equipment, adequate removal and disposal of wastes, and making sure that the people concerned are trained in microbiological methods and acquainted with the tests.

    Importance of ASTM E640 

    ASTM E640 has a significant function in the protection of cosmetics and their efficiency through the assessment of preservatives used in water-soluble formulations. This standard offers a procedure for evaluating preservatives for their ability to perform up to certain levels; this is very important, especially because microbial growth is a significant threat to product quality, extension, and consumer health.

    FAQ's

    Where can I get the astm e640 tested?

    You can share your astm e640 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific astm e640 test to various testing techniques.

    How much do I need to pay for the astm e640 test?

    Please contact us for a detailed quote for your astm e640 testing needs. Cost incurred to carry out different astm e640 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

    How many samples are required for astm e640?

    The required number of samples or specimens should comply with the procedure given in the astm e640 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

    How much discount can I get on the astm e640 test?

    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

    How many days will it take to complete the astm e640 test?

    The turnaround time for astm e640 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the astm e640 tested?

    You can share your astm e640 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific astm e640 test to various testing techniques.

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