Last updated 6th March 2025

ASTM D3273 Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber

ASTM D3273 is utilized to assess fungal defacement on interior coatings in an accelerated manner, focusing on the relative resistance of paint films to mold and mildew propagation under ideal fungal growth conditions. ASTM D3273 offers flexibility to adjust different fungus species for customized projects and provides a straightforward procedure for controlling external variables. Primarily designed for testing paints and varnishes, ASTM D3273 is often complemented by ASTM D3274 for standardized rating systems.

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    ASTM D3273 Introduction

    ASTM D3273 outlines the use of a compact environmental chamber and its operational conditions to reliably assess, over a 4-week period, the relative resistance of paint films to surface mold and mildew growth within a severe interior environment. The apparatus is designed for easy construction or procurement by interested parties and is intended to replicate results obtained in a large tropical chamber. ASTM D3273 serves as a tool for evaluating the comparative resistance of interior coatings to accelerated mildew growth.

    ASTM D3273 Test Method

    Growth Chamber SetupFor ASTM D3273, the growth chamber is prepared with appropriate soil and water content and then stabilized for 24 hours at approximately 32°C.
    IncubationIn ASTM D3273, suspensions containing three different fungi cultures are evenly spread over the soil surface and allowed to incubate for approximately 14 days. Subsequently, non-coated substrates and coated test panels are randomly placed in a sporulating chamber by vertical hanging. 

    D3273 Equipment and Sample Preparation

    Specimen DetailsSoil, softwood, and sapwood test panels, gypsum test panels, drawdown paper, tongue depressors, and glass are used.
    Specimen SizeSoil with 25% peat moss, Sapwood Panels (13 mm thick, 75×100 mm), and Gypsum Board Panels (13-25 mm thick, 75×100 mm).

    D3273 Test Results and Interpretation

    ASTM D3273 evaluates the resistance of interior coatings (such as paints and varnishes) to mold growth on their surfaces within an environmental chamber.

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    ASTM D3273 Testing
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    Tests Related to ASTM D3273

    ASTM D3273 is related to tests,, including ASTM E177, which describes terminology used in ASTM methods, and ASTM E691, which involves conducting lab studies to determine the precision of ASTM tests.

    Use of D3273 in Various Industries

    The standard is used in construction, coatings, and materials science to evaluate mold resistance in interior coatings under controlled conditions. It is essential that manufacturers maintain product performance and aesthetic integrity in humid environments like bathrooms and basements.

    Materials Commonly Tested with D3273

    It tests interior wall coatings, ceiling tiles, drywall panels, coating systems such as paints, varnishes, and sealants for mold inhibition, and composite materials and polymers for durability and suitability for humid or high-moisture environments.

    Common Challenges and Troubleshooting in D3273 Testing

    The environmental chamber conditions, including humidity or temperature fluctuations, make testing challenging and require proper calibration. Troubleshooting also includes uniform application of coatings, sterility verification, and consistent spore suspension concentrations to accurately replicate real-world scenarios.

    Safety and Best Practices in D3273 Testing

    Safety testing involves handling mold spores and disposing of contaminated materials, using personal protective equipment, working in a well-ventilated area, and maintaining chambers regularly to prevent cross-contamination, ensure accurate results, and minimize spore carryover.

    Importance of D3273

    The specification is very important in determining the durability and health safety of interior coatings, especially in mold-prone environments. It ensures product quality, boosts consumer trust, and reduces mold-related health risks that contribute to healthier indoor environments and encourage innovation.

    FAQ's

    Where can I get the astm d3273 tested?

    You can share your astm d3273 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific astm d3273 test to various testing techniques.

    How much do I need to pay for the astm d3273 test?

    Please contact us for a detailed quote for your astm d3273 testing needs. Cost incurred to carry out different astm d3273 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

    How many samples are required for astm d3273?

    The required number of samples or specimens should comply with the procedure given in the astm d3273 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

    How much discount can I get on the astm d3273 test?

    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

    How many days will it take to complete the astm d3273 test?

    The turnaround time for astm d3273 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the astm d3273 tested?

    You can share your astm d3273 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific astm d3273 test to various testing techniques.

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