MaTestLab > ISO Standards > ISO 14853 Anaerobic Biodegradability of Plastic Materials
Last updated 29th May 2025

ISO 14853 Anaerobic Biodegradability of Plastic Materials

ISO 14853 test for plastic material biodegradability in anaerobic aqueous medium. This test measures how many carbons are being transformed from the sample into gaseous carbon, CO2, and CH4 during the biodegradation in the conditions of an anaerobic digester, which also can demonstrate that the product is stable during a defined period required for declaring its biodegradability. The duration of the test can sometimes go as long as 90 days, depending on how fast your products need to inhibit.

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    Introduction to ISO 14853

    ISO 14853 prescribes an analytical method for determining the ability of plastic materials to be biodegraded in an anaerobic aqueous phase. ISO 14853 measures how much carbon is transiting from the sample to the biogas phases of carbon (CO2 and CH4) during biodegradation under a set of conditions associated with anaerobic digester conditions. The Potential to Validate Status at a Given Point in Time that Helps to Achieve Requirements Connected With The Designation Of The Biodegradability Product. The duration of the test can be from 90 days, according to your product needs.

    ISO 14853 Test Method

    Test procedure In this method, biodegradation is determined in an aqueous system by measuring the rate of production of biogas from anaerobic microorganisms. Sludge activity is tracked using several parallel blank and reference controls. Furthermore, three replicate vessels for each group are also prepared. Changes in the pressure of headspace above the vessels OR flux of biogas, and at the close of the test, inorganic carbon is extracted.
    Incubation condition The test substance is added to a sealed container containing digested sludge at a solid concentration between 1 g/L to 3 g/L. This container is then incubated at 35 ± 2ºC for a maximum of 90 days.

    ISO 14853 Results and Interpretation

    The extent of biodegradation is determined from total inorganic carbon and methane-C produced as a percentage of the measured or calculated amount of carbon added as a test substance, with over 70% of degradation for reference within 60 days. The difference in the percentage biodegradation of the reference in the different vessels is less than 20% of the mean value. If inadequate biodegradation has occurred, pH should not exceed the values of 7 ± 1. 

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    ISO 14853 Testing
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    Use of ISO 14853 in Various Industries

    Commonly used in Bioplastics development for packaging, agriculture, and consumer goods, Wastewater and sludge treatment. 

    Materials Commonly Tested with ISO 14853

     ISO 14853 can be applied to natural and/or synthetic polymers, copolymers, or a mixture, and it applies to water-soluble polymers. Additionally, ISO 14853 can be used for plastics that include additives like plasticizers, colorants, and other substances.

    FAQ's

    Where can I get the iso 14853 tested?

    You can share your iso 14853 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific iso 14853 test to various testing techniques.

    How much do I need to pay for the iso 14853 test?

    Please contact us for a detailed quote for your iso 14853 testing needs. Cost incurred to carry out different iso 14853 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

    How many samples are required for iso 14853?

    The required number of samples or specimens should comply with the procedure given in the iso 14853 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

    How much discount can I get on the iso 14853 test?

    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

    How many days will it take to complete the iso 14853 test?

    The turnaround time for iso 14853 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the iso 14853 tested?

    You can share your iso 14853 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific iso 14853 test to various testing techniques.

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