Ultraviolet-Visible Spectroscopy (UV-Vis Spectroscopy)

Ultraviolet-Visible Spectroscopy (UV-VIS Spectroscopy) is an instrumental technique used for qualitative as well as quantitative chemical analysis. UV-VIS spectroscopy measures the absorbance of ultraviolet (UV) and visible light (VIS) radiation by a sample. measuring the concentrations of various compounds in a solution, such as proteins, metals, and organic molecules. UV-VIS Spectroscopy is based on the principle that when light passes through a sample, some of it is absorbed by the sample while some passes through unaffected. The amount of light absorbed depends on the type and concentration of the compound present in the sample. UV-VIS Spectroscopy is used to observe the optical behavior of chemical compounds, identification of various species, and quantification of specific analytes.


Introduction to Ultraviolet-Visible Spectroscopy (UV-Vis Spectroscopy)

Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis spectroscopy) is absorption spectroscopy or reflectance spectroscopy performed in the ultraviolet-visible spectral region. This test can be performed in a solid state or in a solution. UV-Vis spectroscopy is a simple, cost-effective, versatile, non-destructive analytical technique used for a large spectrum of organic compounds and some inorganic species. UV-VIS spectroscopy provides qualitative and quantitative information about a given compound or molecule. UV-VIS spectroscopy is an analytical method that measures the analyte quantity depending on the amount of light received by the sample. The absorption of ultraviolet or visible radiation (200 nm to 800 nm) by a molecule results in transitions between the molecule’s electrical energy levels. The optical and electronic properties of different materials, such as films, powders, monolithic solids, and liquids, can be characterized using this method.

Principle and Methodology of Ultraviolet-Visible Spectroscopy (UV-VIS Spectroscopy)

When a chemical compound absorbs light, some excitation and de-excitation processes of electrons occur in atoms which result in the production of the distinct spectrum. The transition of electrons at various levels by absorption of radiation from ultraviolet to visible region is plotted in a graph. In a testing lab, the basic instrumentation used in a UV-vis spectrometer comprises a light source, diffraction grating, wavelength selector, sample container or cuvette, and a detector. 

The instrumentation required for UV-VIS spectroscopy includes a source of UV or visible light (such as an LED), a detector (such as an optical fiber), and an optical filter (such as an interference filter). Some instruments may also include additional components, such as polarizers or monochromators for more precise measurements. Finally, all instruments must also have calibration standards available for accurate results.

UV-Vis Spectroscopy Set-up

Uses of Ultraviolet-Visible Spectroscopy (UV-VIS Spectroscopy)

UV-VIS spectroscopy has many applications across various disciplines, including medicine, pharmaceuticals, food science, biochemistry, and environmental science. In pharmaceuticals, it can be used to measure active ingredients; in medicine, this technique can be used to measure levels of drugs or metabolites in blood or urine samples; and in food science, it can be used to measure levels of vitamins or minerals present in foods. This technique has been widely used for environmental monitoring purposes, such as measuring levels of pollutants present in water or air samples.

Advantages of Ultraviolet-Visible Spectroscopy (UV-Vis Spectroscopy)

UV-VIS spectroscopy has high sensitivity, that is, it can detect very small amounts of compounds in samples with high accuracy and precision. Also, this technique does not require any special preparation or treatment prior to analysis, making it an ideal choice for many types of samples. Furthermore, UV-VIS spectroscopy can also measure multiple components simultaneously, making it very useful for complex samples such as biological fluids or environmental samples.

Disadvantages of Ultraviolet-Visible Spectroscopy (UV-VIS Spectroscopy)

Some disadvantages of this technique include its limited range (it cannot detect compounds outside its range), its inability to differentiate between closely related compounds with similar absorption characteristics (known as spectral overlap), and its dependency on measuring absorbance rather than emission like other techniques. Background noise from other sources may interfere with measurements if not accounted for properly during instrument setup and calibration procedures.


Where can I get the uv vis tested?

You can share your uv vis testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific uv vis test to various testing techniques.

How much do I need to pay for the uv vis test?

Please contact us for a detailed quote for your uv vis testing needs. Cost incurred to carry out different uv vis testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

How many samples are required for uv vis?

The required number of samples or specimens should comply with the procedure given in the uv vis standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

How much discount can I get on the uv vis test?

MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

How many days will it take to complete the uv vis test?

The turnaround time for uv vis test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

Where can I get the uv vis tested?

You can share your uv vis testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific uv vis test to various testing techniques.

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