MaTestLab > ISO Standards > ISO 16050: 2003 Determination of aflatoxin B1, and the total content of aflatoxins in cereals, nuts, and derived products with the help of High-performance liquid chromatographic method (HPLC)
Last updated 16th June 2025
ISO 16050: 2003 Determination of aflatoxin B1, and the total content of aflatoxins in cereals, nuts, and derived products with the help of High-performance liquid chromatographic method (HPLC)
A procedure utilizing high-performance liquid chromatography (HPLC) is outlined in ISO 16050:2003 for determining aflatoxin B1 as well as the overall concentration of aflatoxins B1, B2, G1, and G2 in cereals, nuts, and products thereof. When consumed in large quantities, aflatoxins made by some molds can contaminate food items and pose serious health concerns to both people and animals. This standard offers instructions and protocols for precisely measuring aflatoxin levels in various foods, guaranteeing food safety and safeguarding the general public's health. A robust analytical method for precisely detecting and measuring aflatoxins in cereals, nuts, and similar goods is high-performance liquid chromatography. It works by separating, identifying, and quantifying specific components within a sample according to their molecular characteristics.
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Introduction to ISO 16050: 2003
Regarding food safety and quality control, ISO 16050:2003 summarizes the standard’s goals, reach, and importance. Because these mycotoxins can pose health risks, it describes how exact methods of determining aflatoxin contamination in grains, nuts, and goods produced from them must be established. When taken through contaminated food and feed, these toxins which have been identified as potent carcinogens, pose serious health concerns to both humans and animals. Aflatoxin contamination can also result in significant financial losses because contaminated food products must be rejected and foodborne illness outbreaks are expensive. The value of ISO 16050:2003 in terms of offering guidelines and procedures for the efficient identification and measurement of aflatoxins, supporting food safety, public health, and the ease of international trade in grains, nuts, and goods derived from them.
ISO 16050: 2003 Test Method
ISO 16050:2003 uses a high-performance liquid chromatography method for determining aflatoxin. Sample preparation, cleanup, chromatography, calibration, sample analysis, quality control, and data reporting are all part of this method.
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ISO 16050:2003 Testing
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Preparation of the testing setup
ISO 16050:2003 uses a high-performance liquid chromatography method in which the sample is extracted and injected into the HPLC system that has been calibrated. Aflatoxin peaks can be identified by recording the chromatogram and using retention periods and distinctive fluorescence characteristics.
Evaluation of the test
Utilizing peak regions and calibration curves, determine the sample’s aflatoxin concentrations. Indicate the results in parts per billion (ppb) or micrograms per kilogram (µg/kg).
ISO 16050: 2003 Test Specimens
Specimen Detail
ISO 16050:2003 uses high-performance liquid chromatography (HPLC) to measure aflatoxin levels in grains, nuts, and products produced from them.
Specimen Size
According to ISO 16050:2003, the specimen size for aflatoxin analysis is variable based on the testing process’s particulars and laboratory protocols. Usually, the sensitivity of the analytical technique is one of the parameters that defines the specimen size.
ISO 16050: 2003 Test Result
The ISO 16050:2003 standard for aflatoxin determination uses high-performance liquid chromatography (HPLC) to provide quantitative data on aflatoxin B1 and total concentration in nuts, cereals, and byproducts. The results are given in parts per billion (ppb) or micrograms per kilogram (µg/kg) and include metadata about chromatographic settings, quality control procedures, extraction and cleanup techniques, and sample identification. These test results protect public health and facilitate international trade in food products, ensuring food safety and quality assurance.
ISO 16050: 2003 Related Tests
ISO 16050: 2003 is related to the ISO 21749: 2007 method for the determination of fumonisins B1, B2, and B3 in cereals and cereal products using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). It is also related to the ASTM E2991-17 standard test method for evaluating response robot mobility.
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Please contact us for a detailed quote for your iso 16050 2003 testing needs. Cost incurred to carry out different iso 16050 2003 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.
How many samples are required for iso 16050 2003?
The required number of samples or specimens should comply with the procedure given in the iso 16050 2003 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.
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How many days will it take to complete the iso 16050 2003 test?
The turnaround time for iso 16050 2003 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.
Where can I get the iso 16050 2003 tested?
You can share your iso 16050 2003 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific iso 16050 2003 test to various testing techniques.
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