Last updated 26th May 2025

OECD 207 Earthworm Acute Toxicity Test

The OECD 207 biodegradation test serves as the standard procedure for assessing the toxicity of substances to earthworms. It involves two separate tests: a filter paper contact test and an artificial soil test with Eisenia foetida. The filter paper contact test is more of a rapid screening test, while the artificial soil test provides exposure data to chemicals in soil that is more realistic.

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OECD 207 Introduction

OECD 207 is a standardized test created to evaluate different chemicals’ acute toxicity to earthworms (in particular Eisenia fetida or Eisenia andrei), which are significant to the ecological functions of soil. The test is helpful to assess possible risks to soil-dwelling organisms from pesticides, industrial chemicals, etc.

OECD 207 Test Method

This test is performed in two categories; one with paper contact toxicity test which is a rapid test and another artificial soil testing which takes a longer duration of time. 

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OECD 207 Testing
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Test procedure for the paper contact toxicity testFor this test, the earthworms are exposed to the test substance on a moist filter paper. This helps in identifying the potential toxic chemical.
Test procedure for artificial soil testingIn this the earthworms are kept sample precisely defined artificial soil during the duration of test. This determines and gives the data of natural exposure of earthworms to chemicals. 

OECD 207 Equipment and Sample Preparation

Specimen Details for Filter Paper Contact Toxicity Test. The testing range employs five different dosages, ranging from 0.0001 mg/cm² to 1.0 mg/cm². For each dosage, ten replicates are required, with each vial containing a single worm. Each dosage requires ten replicates, and each vial holds a single worm. The vials are placed on their sides in the dark at a temperature of 20º ± 2ºC for 48 hours.
Specimen Details for Artificial Soil TestTo conduct this procedure, five different concentrations of the test substances arranged in a geometric series should be utilized, ensuring that one concentration leads to no deaths while another concentration causes complete mortality. These treatments are to be repeated four times, and the mortality rates of the earthworms will be evaluated both 7 days and 14 days after application.

OECD 207 Results and Interpretation

The mortality of the earthworms after 14 days are measured. Then the Lethal Concentration dose (LCD 50) is determined. Secondly if there are any abnormalities , those are analysed in earthworms. 

Tests Related to OECD 207

OECD 222 (Earthworm Reproduction Test) – evaluates the long-term impacts.  

OECD 317 (Bioaccumulation in Terrestrial Oligochaetes) – quantifies the absorption.  

ISO 11268-1 determines the acute toxicity using artificial soil substrate. ISO 11268-2 determines the effect of pollutants on earthworm reproduction. 

FAQ's

Where can I get the oecd 207 tested?

You can share your oecd 207 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 207 test to various testing techniques.

How much do I need to pay for the oecd 207 test?

Please contact us for a detailed quote for your oecd 207 testing needs. Cost incurred to carry out different oecd 207 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

How many samples are required for oecd 207?

The required number of samples or specimens should comply with the procedure given in the oecd 207 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

How much discount can I get on the oecd 207 test?

MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

How many days will it take to complete the oecd 207 test?

The turnaround time for oecd 207 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

Where can I get the oecd 207 tested?

You can share your oecd 207 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 207 test to various testing techniques.

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