The ASTM E579 test method measures the sensitivity of a fluorescence measuring system by determining the limit of detection (LOD) of quinine sulfate dihydrate in solution. The excitation and fluorescence bands are kept broader than 10 nm for performance evaluation. SI units are considered the standard of measurement.
ASTM E579 Standard Test Method for Limit of Detection of Fluorescence of Quinine Sulfate in Solution
What is ASTM E579?
What is the Scope of ASTM E579?
ASTM E579 test method determines the limit of detection of a fluorescence substance by using the technique of molecular fluorescence spectroscopy. It helps specify applications for fluorescent materials. The data obtained can be used for research, quality control, and performance evaluation. The readings are taken with respect to the signal-to-noise ratio to ensure accuracy. The results are employed to make effective fluorescence measuring systems.
ASTM E579 Sample Preparation Guide
Specimen size should be as per the requirements. In this case, the solution taken for the performance of the test weighs 0.100g.
ASTM E579 Testing Procedure and Requirements
The test apparatus is set up to determine the limit of detection of quinine sulfate dihydrate in solution. The excitation and emission bandwidth for the fluorescence measuring system is adjusted. The peak wavelengths are set to be 350 and 450 nm, respectively. The solvent is put in the sample cell, and readings for the signal are noted. The ten consecutive measurements are taken, and the final result is expressed by taking their average.
ASTM E579 Analysis Results and Interpretation
The limit of detection for the specimen is determined by means of signal-to-noise ratio, where S represents the difference between readings and N is the total root mean square noise.
Updated on August 24, 2026
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