By content81c70d6e36 | Last updated 18th June 2025

ASTM D5864 Biodegradability of Lubricants by CO₂ Evolution

ASTM D5864 is test for aqueous aerobic biodegradability evaluates the biodegradability of lubricants and their constituents by tracking CO₂ production in bottles that are continuously aerated. This approach is intended for lubricants that do not possess volatile properties or inhibit the inoculum.

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    Introduction to ASTM D5864

    ASTM D5864 is a standardized procedure established by ASTM International to assess the aerobic biodegradability of lubricants in aqueous environments. It specifically targets lubricating oils, greases, and hydraulic fluids to evaluate their degradation potential when they enter natural water ecosystems. The method employs microorganisms in oxygen-abundant conditions and quantifies the CO₂ emissions, reflecting the extent of lubricant degradation through microbial processes.

    ASTM D5864 Test Method

    Test procedure The lubricant samples are exposed to activated sludge from a municipal wastewater treatment plant, which serves as the inoculum in this test under laboratory conditions, and the level of CO2 evolved is measured.

    ASTM D5864 Equipment and Sample Preparation

    Specimen DetailsGlass vessels are filled with a suitable medium and the sample. The CO2 production is determined using the control for up to 28 days. 

    ASTM D5864 Results and Interpretation

    The final results are expressed as a percentage of theoretical CO₂ evolution. Based on the standard: 

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    1. A lubricant is deemed readily biodegradable if it reaches at least 60% biodegradation within 28 days. 
    2. If a substance does not satisfy this criterion, it could still be inherently biodegradable, but additional testing is required.

    Use of ASTM D5864 in Various Industries

    This test is usually utilized in the Marine and shipping industries, Forestry and agriculture, Construction and mining, and Automotive and aviation sectors

    Common Challenges and Troubleshooting in ASTM D5864 Testing

    The common challenges are that poor dispersion of lubricant in water can delay biodegradation, toxic additives in the lubricant may inhibit microbial activity, and interference in CO₂ trapping can cause inaccurate gas measurements.

    FAQ's

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    How many samples are required for astm d5864?

    The required number of samples or specimens should comply with the procedure given in the astm d5864 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

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    How many days will it take to complete the astm d5864 test?

    The turnaround time for astm d5864 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the astm d5864 tested?

    You can share your astm d5864 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific astm d5864 test to various testing techniques.

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