ASTM D3273 Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber

    What is ASTM D3273?

    ASTM D3273 is a standard test method for determining the relative resistance of interior coatings to surface fungal growth in a severe interior environment. This quick test uses small environmental chambers to evaluate mold and mildew propagation on paint films after 4 weeks. The method is intended to test interior paints, varnishes, and other coatings in moist or humid areas. ASTM D3273 (Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber) uses controlled temperature and humidity to encourage rapid mold growth. The apparatus is designed for easy construction or procurement by any interested party and duplicates results obtained in large tropical chambers.

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    Get Certified ASTM D3273 Testing for Reliable Mold Resistance Assessment

    Interior coatings exposed to mold can discolor, bubble, peel, and develop a musty smell. ASTM D3273 testing guarantees paints and coatings are durable in moist environments. Qualified laboratory testing gives precise and repeatable data on quality control, product development, and comparative assessment. This testing assists manufacturers in creating coatings that are mould-resistant and promotes consumer confidence in products for use in bathrooms, kitchens, and basements. 

    What is the Scope of the ASTM D3273 Test Standard?

    ASTM D3273 tests the ability of interior coatings to resist the growth of mold. The standard applies to the relative performance of paint films and coatings against surface defacement with fungi under accelerated conditions in an environmental chamber. Laboratories may test coated panels or coating systems using these methods. The standard specifies test conditions but does not give absolute acceptance criteria; comparative performance data is provided. 

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    The scope of ASTM D3273 includes: 

    • Material type: ASTM D3273 tests relative resistance to surface mold and mildew growth of interior coatings. 
    • Purpose: ASTM D3273 evaluates the relative resistance of interior coatings to surface mold and mildew growth.
    • Property measured: The selected test is a measure of the percentage of fungal growth coverage of the surface of the coated panel. 
    • Method: The environmental chamber in ASTM D3273 is set to a temperature of 32.5 ± 1 °C with a relative humidity above 93 % to encourage the growth of moulds. 
    • Result: The testing laboratory receives comparative performance ratings of the coating formulations it tests. 
    • Test Limitations: The standard does not set acceptance criteria or specific service life for every system, as data is provided for comparison. 
    • Environmental conditions: Fungal growth conditions are incorporated into the test program, with controlled temperature and humidity. 
    • Applications: Engineers use ASTM D3273 to evaluate interior coatings for use in moisture-prone environments such as bathrooms, kitchens, and basements. 

    What are the Uses of ASTM D3273 Testing?

    ASTM D3273 testing assists laboratories in providing accurate data for coating quality evaluation. The results are utilized by manufacturers and engineers in product development, quality control, and selection of materials. This standard-

    • Assessing mould resistance of interior paints and coatings
    • Evaluation of antifungal additives and total levels for use
    • Examine different coating products for moist/humid conditions
    • Assisting with bathroom, kitchen and basement product development
    • Data to control quality and ensure manufacturing consistency
    • For coatings intended for use in an interior environment where mold is a potential problem, the estimation of performance needs to take into consideration the conditions that favor mold growth. 

    Which materials can be tested under ASTM D3273?

    ASTM D3273 deals with interior coatings such as sealants, varnishes and paints. The test method is suitable for evaluating coating systems on various substrates such as gypsum board, wood panels, and other interior building materials. The standard is designed for use to test interior coating systems and is not yet validated for exterior coatings. 

    Why is ASTM D3273 Important?

    ASTM D3273 is an accelerated test to evaluate the resistance of the interior coating to mold growth. Painted surfaces may develop mold that will affect the integrity of the paint, resulting in discoloration, bubbling, peeling of painted surfaces, and musty odors. This standard provides a means for manufacturers to create mold-resistant coatings, and for consumers to choose products for use in moist environments. The outcome helps engineers select coatings and to decide if a coating will achieve acceptable performance in interior conditions conducive to mold growth. For every rating, a better-performing coating will generally perform better in actual use, but the performance for any rating does not necessarily mean any specific period of time for a fungus-free coating. 

    ASTM D3273 Equipment and Sample Preparation Guide

    ASTM D3273 requires an environmental chamber that holds controlled temperature and humidity. Samples are prepared on appropriate substrates before testing. 

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    Sample and Specimen Details Technicians apply representative samples of interior coatings to test panels and prepare them for controlled environmental chamber exposure. 
    Specimen PreparationTechnicians apply coating materials uniformly to test panels such as gypsum board or wood and allow proper drying before testing. 
    Specimen DimensionsTest panels typically measure 75 × 100 mm with thickness ranging from 13 to 25 mm depending on substrate type. 
    Instrumentation
    The test uses an environmental chamber with temperature control (32.5 ± 1 °C), humidity control (>93 % RH), and incubation facilities. 

    Testing Procedures and Requirements for ASTM D3273

    Mold resistance is assessed over 4 weeks using an ASTM standard accelerated method in an environmental chamber. The standard that the test employs is in SI units. The approach is as follows: 

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    Chamber Preparation Technicians place soil with appropriate moisture content in the chamber and stabilize it at 32.5 ± 1 °C for 24 hours. 
    Fungus Inoculation Technicians spread suspensions containing three different fungal cultures evenly over the soil surface. 
    Incubation The chamber incubates for approximately 14 days to establish fungal growth. 
    Panel Placement Technicians randomly place coated test panels in the sporulating chamber by vertical hanging. 
    Exposure Period Panels remain in the chamber for 4 weeks at >93 % relative humidity. 
    Evaluation Technicians inspect panels weekly and rate them according to the ASTM D3273 grading scale. 

    ASTM D3273 Testing Process and Data Collection

    The testing process starts with preparing the soil and water content in the environmental chamber. The chamber temperature is set to 32.5 ± 1 °C and maintained for 24 hours. The suspensions have three fungal cultures and are spread evenly over the soil surface. The chamber is incubated for about 14 days to let the fungi grow and establish. Test panels are coated with coating material, then randomly hung vertically in the sporulating chamber. The exposure is continued for 4 weeks in controlled conditions. The panels are examined weekly, and the percentage of the surface covered with fungal growth is recorded following the ASTM D3273 grading system.

    The image depicts an environmental chamber used for evaluating mold resistance of interior coatings according to ASTM D3273.
    ASTM D3273 Environmental Chamber for Mold Resistance Testing

    Common Challenges and Troubleshooting in ASTM D3273

    Precise control of environmental conditions is essential to achieve accurate ASTM D3273 results. Reproducible results require tight control of temperature and humidity within the specified range. Mold growth on a film depends on the moisture level of the film and substrate. Issues that are common are humidity oscillations, temperature changes, contamination, and spore suspension concentration. High-quality results are obtained in the laboratory as a result of careful calibration of chambers, verification of sterility, application of even coating, and trained technicians who perform the tests according to standard procedures.

    ASTM D3273 Analysis Results and Interpretation

    For ASTM D3273, the results are reported in the standard grading scale. Panels are examined each week for fungal growth and then assigned a grade based on the ASTM D3273 grading system:

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    • The laboratory reports the fungal-growth rating on a scale from 0 to 10, with higher ratings indicating a greater percentage of surface remaining clear of fungal growth.
    • Analysts assign a rating of 10 when the panel shows no fungal growth and 0 when fungal growth covers the entire evaluated surface.
    • Technicians evaluate the panels at the specified weekly intervals and records the percentage of surface affected by fungal growth.
    • Engineers interpret intermediate ratings based on the percentage of surface that remains clear, with each rating corresponding to a 10% increment in clear surface.
    • Analysts compare fungal-growth ratings among coating specimens to evaluate differences in resistance to fungal growth under the specified test conditions.

    Link to ASTM D3273

    FAQ

    What are the test conditions of the ASTM D3273?
    To encourage and sustain mold growth on test panels, ASTM D3273 specifies controlled conditions of 32.5 ± 1 °C and relative humidity greater than 93 %.
    The ASTM D3273 rating system is from 0 to 10, with 10 being no fungal growth and 0 being 100 % surface coverage by mold. The numbers indicate the percentage of the surface of the items that remain clear: 9 means 90 % remain clear, and 0 means 0 % remain clear.
    ASTM D3273 is designed to perform accelerated testing of interior coatings. It has not been proven to assess the performance of exterior coatings, and limitations have not been established for exterior coatings.

    Updated on September 14, 2026

    Gokula Srinivasan Selvam
    About Author
    Gokula Srinivasan Selvam is a Material Testing Associate at MaTestLab Inc. and holds a postgraduate degree in Ceramic Engineering from IIT (BHU), Varanasi.
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