SELDI is the acronym for Surface-Enhanced Laser Desorption/Ionization, representing a method of high-order mass spectrometry applied to the analysis of complex biological materials. It increases analyte ionization by virtue of interaction with a specific surface, which is useful in detecting and quantifying low-abundance biomolecules. A particular power of SELDI in proteomics comes from its ability to directly profile protein mixtures from native biological fluids and tissues.
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Introduction to Surface-Enhanced Laser Desorption/Ionization
SELDI is a technology that combines surface enhancement with classical MALDI to improve sensitivity and specificity in the detection of biomolecules. Surface-Enhanced Laser Desorption/Ionization was developed in the late 1990s and has contributed largely to biomarker development processes, diagnosis of diseases, and treatment monitoring. It has become an integral tool in clinical proteomics due to its ability to analyze proteins, peptides, and other biomolecules in complex mixtures with very little sample preparation. MaTestLab is one of the leading testing service providers in the USA and Canada. We have a large network of testing laboratories in the USA.
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Principle and Methodology of SELDI
SELDI is a technique that allows for high laser desorption/ionization efficiency. This is possible through the selective binding of analytes to a modified surface from a complex sample matrix. The steps involved in the process include sample preparation, binding, laser desorption/ionization, mass spectrometry analysis, and data analysis. Biological samples can be either blood, urine, or tissue extracts, which are prepared and applied to the surface with prescribed chemical properties. The molecules are ionized and introduced to a mass spectrometer for analysis, which generates a spectral profile.
Instrumentation
The SELDI chip is a component of the laser analysis system and is derivatized with various chemical groups to define what types of analytes will bind to it. The bound analytes are desorbed and ionized by pulses of UV radiation. The parameters of this process can be changed for efficiency. A mass spectrometer analyzes the ionized molecules according to the time-of-flight technology to identify the m/z ratio. Thereafter, specialized software processes the mass spectra, identifies peaks, and conducts quantitative and qualitative analysis. Advanced bioinformatics tools are at liberty to interpret data and correlate this information with biological functions or even a disease state.
Applications of Surface-Enhanced Laser Desorption/Ionization (SELDI)
SELDI represents a potential platform for the discovery of biomarkers, diagnostics of diseases, therapeutic monitoring, research in proteomics, and drug development. The diseases associated with proteins that Surface-Enhanced Laser Desorption/Ionization helps identify range from cancer to Alzheimer’s disease and cardiovascular conditions, among many others. It also helps in early diagnosis and, hence, tracking of the level of biomolecules over time for personalized treatment. Surface-Enhanced Laser Desorption/Ionization will also help in proteomics research to understand the molecular mechanisms and hence the drug targets.
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FAQ's
Where can I get the seldi tested?
You can share your seldi testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific seldi test to various testing techniques.
How much do I need to pay for the seldi test?
Please contact us for a detailed quote for your seldi testing needs. Cost incurred to carry out different seldi testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.
How many samples are required for seldi?
The required number of samples or specimens should comply with the procedure given in the seldi standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.
How much discount can I get on the seldi test?
MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.
How many days will it take to complete the seldi test?
The turnaround time for seldi test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.
Where can I get the seldi tested?
You can share your seldi testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific seldi test to various testing techniques.
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