Introduction to Desorption Electrospray Ionization
DESI: Mass spectrometry (MS) is an advanced analytical tool for the determination of chemical substance composition. Most classical ionization techniques under MS usually demand very specialized sample preparation and operation in a vacuum. DESI provides a really strong turn in this approach by making materials get ionized directly in their native state. In doing so, DESI presents a fast and clean method of analysis, enhancing mass spectrometry applications in the real world. MaTestLab is one of the leading testing service providers in the USA and Canada. We have a large network of testing laboratories in the USA.
Principle and Methodology of Desorption Electrospray Ionization
DESI is a technique that utilizes charged droplets formed as a result of an electrospray process in analyte molecule desorption and ionization on the surface of a sample. The technique consists of the process of high-voltage power supply, droplet impact, desorption and ionization, and finally, the collection of ions. The ions are further captured and analyzed by a mass spectrometer. The process involves supply from a high voltage, droplet impact, and finally, ion collection, which allows for correct analysis of the surface of the sample under consideration.
Instrumentation
The instrument consists of three main parts—an electro-spray source, a sample stage, and a mass spectrometer. With the help of a capillary and high voltage power supply, the electro-spray source generates charged solvent droplets. The sample is mounted on the movable stage to determine an optimal droplet impact angle and distance. TOF, quadrupole, and ion trap mass spectrometers are coupled for analysis.
Applications of Desorption Electrospray Ionization (DESI)
DESI is a versatile and very sensitive sample preparation technique applied in forensic science, the pharmaceutical industry, environmental monitoring, and clinical diagnostics. It allows the investigation of trace amounts of drugs, explosives, and other substances directly off surfaces such as skin, garments, and paper—very useful in any criminal investigation. In the pharmaceutical industry, it allows for the fast screening of drugs across varied surfaces. On its part, DESI helps to detect environmental pollutants and contaminants from soil, water, and air samples.
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