ISO 23977-1 Aerobic Biodegradability of Plastics in Seawater by Measuring CO2 Evolution

    Introduction to ISO 23977-1

    Plastic pollution in the aquatic environment has become a worldwide problem. In response to this, scientifically tested materials that are supposed to be biodegradable in water should be considered. The ISO 23977-1 mandates a method for predicting the complete aerobic biodegradability of plastics in waterborne processes via natural microbial inocula to mimic possible environmental scenarios. According to the ISO 23977-1 standard, particular relevance is due to marine and freshwater-safe plastics, especially for single-use (e.g., packaging, disposable cutlery and utensils) and biodegradable fishing gear, which has a characteristic to be mismanaged in the aquatic environment.

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    ISO 23977-1 Test Method

    Test procedure This test is executed at mesophilic test conditions for not more than 2 years. Plastic materials are therefore incubated only with seawater, a pelagic seawater test, or seawater of low sediment concentration added ( suspended sediment seawater test). The ultimate test of biodegradation in plastics is the amount of CO2 produced from the biodegradation of the plastic material.

     ISO 23977-1 Sample Preparation 

    Test procedure The sample size should be large enough for an accurate measurement of the CO2 evolution and should not overwhelm the seawater and impact the test negatively. 

    ISO 23977-1 Results and Interpretation

    The amount of CO2 evolved is compared to the theoretical amount of evolved CO2 and expressed as a percentage. This yielded the maximum level of biodegradation.

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    Use of ISO 23977-1 in Various Industries

    Generally used in the plastic industry and determines the plastic presence in seawater.

    Materials Commonly Tested with ISO 23977-1

    Primarily used for testing plastics. This principle and methodology of ISO 23977-1 can be adapted for other materials like composites or bioplastics.

    Updated on October 12, 2026

    Divakar Shukla
    About Author
    Divakar Shukla is an Electrical and Electronics Engineer specializing in the convergence of embedded systems architecture, industrial automation, and applied artificial intelligence.
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