ISO 16221 Seawater Biodegradability Test

    Introduction to ISO 16221

    With the increasing awareness of the environment, biodegradable and compostable plastics are in demand more than ever. But as the “green” marketing claims have increased, so too have the grey areas, leading to extensive cases of greenwashing in what should be an honest standard of environmentally friendly products. In this respect, ISO 16221 was created to provide a scientifically sound framework for statements claiming biodegradability. The regulation helps manufacturers, regulators, and consumers with criteria of clear labeling and enforcement, to some extent, that the biodegradability is based only on an applicable testing standard. 

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    ISO 16221 Test Method

    There are five methods in this ISO method for freshwater, which are altered to suit marine biodegradation. 

    Test procedure for DOC die-away test.  Along with ISO 7827, this test evaluates the ready and ultimate aerobic biodegradability of organic compounds through the analysis of dissolved organic carbon (DOC).
    Test procedure for Closed BottleThis test is for the ultimate aerobic biodegradability of organic compounds through biochemical oxygen demand analysis.
    Test Procedure for Two-Phase Closed BottleThis test determines the ultimate aerobic biodegradability of organic compounds through the determination of biochemical oxygen demand in a two-phase closed bottle test.
    Test procedure for CO2 Evolution This test evaluates the ultimate aerobic biodegradability of organic compounds by measuring the CO2 evolution.
    Test procedure for the CO2 Headspace TestThis test evaluates the ultimate aerobic biodegradation of organic compounds by analyzing the inorganic carbon in sealed vessels.

    ISO 16221 Results and Interpretation

    The results of such tests, when referenced against a reference substance (such as an organic compound of known biodegradability), are useful in determining the ultimate biodegradability of a test substance in the marine environment. The greater the percentage of biodegradation, expressed as the organic carbon converted to CO2 or the oxygen uptake, the higher the biodegradability.

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    Use of ISO 16221 in Various Industries

    ISO 16221 is used in industries dealing with environmental aspects, particularly for testing the biodegradability of substances and waste in marine environments

    Materials Commonly Tested with ISO 16221

    This test is used on materials that are water-soluble, poorly water-soluble (with special measures), volatile, and not inhibitory to the test microorganisms.

    Updated on September 17, 2026

    Divakar Shukla
    About Author
    Divakar Shukla is an Electrical and Electronics Engineer specializing in the convergence of embedded systems architecture, industrial automation, and applied artificial intelligence.
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