ISO 14853 Anaerobic Biodegradability of Plastic Materials

    Introduction to ISO 14853

    ISO 14853 prescribes an analytical method for determining the ability of plastic materials to be biodegraded in an anaerobic aqueous phase. ISO 14853 measures how much carbon is transiting from the sample to the biogas phases of carbon (CO2 and CH4) during biodegradation under a set of conditions associated with anaerobic digester conditions. The Potential to Validate Status at a Given Point in Time that Helps to Achieve Requirements Connected With The Designation Of The Biodegradability Product. The duration of the test can be from 90 days, according to your product needs.

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    ISO 14853 Test Method

    Test procedure In this method, biodegradation is determined in an aqueous system by measuring the rate of production of biogas from anaerobic microorganisms. Sludge activity is tracked using several parallel blank and reference controls. Furthermore, three replicate vessels for each group are also prepared. Changes in the pressure of headspace above the vessels OR flux of biogas, and at the close of the test, inorganic carbon is extracted.
    Incubation condition The test substance is added to a sealed container containing digested sludge at a solid concentration between 1 g/L to 3 g/L. This container is then incubated at 35 ± 2ºC for a maximum of 90 days.

    ISO 14853 Results and Interpretation

    The extent of biodegradation is determined from total inorganic carbon and methane-C produced as a percentage of the measured or calculated amount of carbon added as a test substance, with over 70% of degradation for reference within 60 days. The difference in the percentage biodegradation of the reference in the different vessels is less than 20% of the mean value. If inadequate biodegradation has occurred, pH should not exceed the values of 7 ± 1. 

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    Use of ISO 14853 in Various Industries

    Commonly used in Bioplastics development for packaging, agriculture, and consumer goods, Wastewater and sludge treatment. 

    Materials Commonly Tested with ISO 14853

     ISO 14853 can be applied to natural and/or synthetic polymers, copolymers, or a mixture, and it applies to water-soluble polymers. Additionally, ISO 14853 can be used for plastics that include additives like plasticizers, colorants, and other substances.

    Updated on September 17, 2026

    Divakar Shukla
    About Author
    Divakar Shukla is an Electrical and Electronics Engineer specializing in the convergence of embedded systems architecture, industrial automation, and applied artificial intelligence.
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