OECD 311 Anaerobic Biodegradability of Organic Compounds in Digested Sludge

    Introduction of OECD 311

    Organic content in sewage sludge digestion circumstances, anaerobic degradation is measured by the OECD 311 test guideline. OECD 311 test and unlike the aerobic biodegradation tests (OECD 301 series), the other will not determine if something can degrade in oxygen-free environments, e.g., anaerobic digesters, sediments, and groundwater. The global performance characteristic of anaerobic biodegradation is biogas production, mainly methane (CH₄) and carbon dioxide(CO₂). It is a critical aspect of the understanding of chemicals in wastewater treatment plants and natural anaerobic environments as a whole if chemicals degrade anaerobically.

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    Test Method of OECD 311

    Test procedure In this test, the amount of carbon (inorganic and methane) that developed from the biodegradation of the test substance is determined by measuring net gas production and net inorganic carbon development in the liquid phase (considering the blank control values). The percentage of biodegradation is determined as the amount of total inorganic carbon and methane-C developed with the measured or estimated amount of carbon from the test substance.

    OECD 311 Sample Preparation

    Specimen DetailsThe washed digested sludge, containing low concentrations of inorganic carbon (<10 mg/L), is diluted to achieve a total solids concentration between 1 g/L and 3 g/L. The test substance is added to the prepared sludge at concentrations ranging from 20 to 100 mg of carbon per liter. 
    Incubation conditionThe mixture is then incubated in sealed vessels at a constant temperature of 35 ± 2°C for up to 60 days.

    OECD 311 Results and Interpretation

    After this testing, biogas production is measured as the increase in headspace pressure in the sealed containers. The inorganic carbon concentration in the liquid phase is also measured at the end of the experiment. Biodegradation is calculated by taking the total amount of carbon produced (in methane and inorganic carbon) and expressing it as a percentage of the initial carbon content of the test material. A high percentage of conversion of carbon into biogas indicates that the test material is readily biodegradable under anaerobic conditions. A lower percentage indicates that the anaerobic biodegradability may be limited, and further environmental risk assessments may be needed.

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    This test is similar to ISO 11734. 

    Materials Commonly Tested with OECD 311

    This test is suitable for evaluating the anaerobic biodegradability of a wide range of organic compounds, including those found in detergents, surfactants, and industrial chemicals.

    OECD 316 determines the chemical that impacts the aquatic environment. OECD 320  determines the anaerobic transformation of chemicals in liquid manure. 

    FAQ

    Where can I get the oecd 311 tested?
    You can share your oecd 311 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 311 test to various testing techniques.
    Please contact us for a detailed quote for your oecd 311 testing needs. Cost incurred to carry out different oecd 311 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.
    The required number of samples or specimens should comply with the procedure given in the oecd 311 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.
    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.
    The turnaround time for oecd 311 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.
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