The OECD 301 F test is one of the tests in the OECD 301 series to determine the ready biodegradability of a chemical, where biodegradation occurs in an aerobic environment. Known as the Manometric Respirometry Test. This test assesses oxygen uptake by microorganisms during the degradation of a test substance and the determination of true biodegradability.
Test Procedure of OECD 301F
| Test procedure | A closed bottle contains some water and the sample material, creating a small air space inside. As the sample breaks down, it uses up both the oxygen and the air space. The drop in oxygen pressure is quantified using a manometer, which serves as a measure of the biodegradation rate. |