OECD 301B CO2 Evolution Ready Biodegradability Test; Modified MITI TEST(I)

    OECD 301B Introduction

    The OECD 301B test is part of the OECD 301 test series, which is designed to measure the aerobic biodegradation or readiness for biodegradation of a chemical substance, where the substance is placed in a closed system containing a microbial inoculum, and the loss of the substance is measured through the production of CO2. This method uses respirometry to measure a material´s ability to biodegrade based on the measurement of CO2 production in a liquid medium for at least 28 days.

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    OECD 301B Test Method

    Test procedure In this test the aerobic biodegradability is analysed using respirometry and the amount of CO2 emission is determined after an incubation period of 28 days in the closed vessels.

    OECD 301B Sample Preparation

    Specimen DetailsThe concentration of the test substance must be around 10-20 mg carbon/L, with the temperature of 22 ± 2℃ and pH between 6-8. 

    OECD 301B Results and Interpretation

    At the end of the incubation period of 28 days the  percentage CO2 evolved in the vessels is determined. And depending on the emission, the ready biodegradability of the sample is analysed. Usually 60% CO2 emission in an 8-10 days window is considered readily biodegradable. 

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    Materials Commonly Tested with OECD 301B

    This method is used for testing water soluble, non-volatile organic chemicals such as detergents, surfactants, pesticides, pharmaceuticals, industrial chemicals and personal care products. 

    OECD 303 A-C series determines the biodegradation in different environments, including soil. 

    FAQ

    Where can I get the oecd 301b tested?
    You can share your oecd 301b testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific oecd 301b test to various testing techniques.
    Please contact us for a detailed quote for your oecd 301b testing needs. Cost incurred to carry out different oecd 301b testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.
    The required number of samples or specimens should comply with the procedure given in the oecd 301b standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.
    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.
    The turnaround time for oecd 301b test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.
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