By content81c70d6e36 | Last updated 16th June 2025

 ISO 16634-2:2016 Determination of the total nitrogen content by combustion according to the Dumas principle and calculation of the crude protein content

The Dumas combustion method is a technique described in ISO 16634-2:2016 for determining the total nitrogen content in food and animal feed. To transform nitrogen into nitrogen gas, which is then measured, the sample must be burned in an oxygen-rich atmosphere. A conversion factor unique to the type of food or feed is used to determine the crude protein concentration based on the total nitrogen content. For quality assurance and legal compliance, ISO 16634-2:2016 guarantees accurate and uniform outcomes in nutritional analysis.

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    Introduction to ISO 16634-2:2016

    According to the Dumas principle, ISO 16634-2:2016 provides a standardized combustion procedure to ascertain the total nitrogen concentration of food products and animal feeding materials. This technique is essential for precisely determining crude protein concentration, a necessary indicator for nutrition labels and quality assurance in the feed and food industries. 

    The nitrogen in the sample is transformed into nitrogen gas, which is subsequently measured by burning it in an oxygen-rich atmosphere. This standard surpasses conventional techniques like the Kjeldahl method in ensuring excellent accuracy, safety, and efficiency in the detection of nitrogen and protein contents. The use of ISO 16634-2:2016 supports regulatory compliance and quality assurance in the production of food and feed by facilitating consistency and reliability in nutritional analysis.

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    ISO 16634-2:2016 Test Method

    The test method for determining the total nitrogen content and calculating the crude protein content according to ISO 16634-2:2016 involves several key steps, including sample preparation, combustion, and reduction of nitrogen oxides, detection, measurement, and calculation. This technique is used to determine the nitrogen and protein content of different food products and animal feeds because of its great precision, safety, and speed.

    Preparation of the testing setupThe combustion device is installed correctly and according to the manufacturer’s instructions. This involves ensuring that everything is securely fastened and positioned steadily to avoid vibrations and other disruptions while it is operating. 
    Evaluation of the testUtilize the reduction furnace to transform the nitrogen oxides created during combustion into nitrogen gas (N2). Utilizing the thermal conductivity detector (TCD), determine the nitrogen gas content. Note the nitrogen gas production amount as indicated by the detector readings.

    ISO 16634-2:2016 Test Specimens

    Specimen DetailAccording to ISO 16634-2:2016, a test specimen should be a sample indicative of the full batch of food product or animal feed.
    Specimen SizeThe sample size is sufficient for multiple analyses. It is usually advised to use at least 100 grams for homogenization and further testing.

    ISO 16634-2:2016 Test Result

    Test specimen analysis in compliance with ISO 16634-2:2016 yields important information on nutritional composition, which is mainly reported as crude protein content and total nitrogen content. These findings, which guarantee accuracy, precision, and dependability, are essential for quality control, nutritional labeling, and regulatory compliance in the food and feed sectors.

    ISO 16634-2:2016 Related Tests

    ISO 16634-2:2016 is related to the ISO 16634-1:2008 determination of nitrogen content using the Kjeldahl method and to ISO 1871:2009 general guidelines for the determination of nitrogen by the Kjeldahl method.

    FAQ's

    Where can I get the iso 16634 2 2016 tested?

    You can share your iso 16634 2 2016 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific iso 16634 2 2016 test to various testing techniques.

    How much do I need to pay for the iso 16634 2 2016 test?

    Please contact us for a detailed quote for your iso 16634 2 2016 testing needs. Cost incurred to carry out different iso 16634 2 2016 testing methodology depends on the type of raw material; number of samples, coupons, or specimens; test conditions, turn around time etc. Costs of some ASTM testing methods start from $100 and the final value depends upon the factors listed above. Please contact us for the best and latest prices.

    How many samples are required for iso 16634 2 2016?

    The required number of samples or specimens should comply with the procedure given in the iso 16634 2 2016 standard. However, the MaTestLab operations team can assist you for your special requirements once you share your testing details with us.

    How much discount can I get on the iso 16634 2 2016 test?

    MaTestLab has a vast testing laboratory network, hence we bring you the best testing facilities in a cost-effective way. We offer considerable discounts (15-20%) to our returning customers based on test volume and frequency.

    How many days will it take to complete the iso 16634 2 2016 test?

    The turnaround time for iso 16634 2 2016 test methodology depends upon the test procedure mentioned in the standard test document. However, we at MaTestLab understand your research requirements and hence try to get your test completed within the least possible time.

    Where can I get the iso 16634 2 2016 tested?

    You can share your iso 16634 2 2016 testing requirements with MaTestLab. MaTestLab has a vast network of material testing laboratories, spread across the USA and Canada. We support your all material testing needs ranging from specific iso 16634 2 2016 test to various testing techniques.

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