According to the Dumas principle, ISO 16634-2:2016 provides a standardized combustion procedure to ascertain the total nitrogen concentration of food products and animal feeding materials. This technique is essential for precisely determining crude protein concentration, a necessary indicator for nutrition labels and quality assurance in the feed and food industries.
The nitrogen in the sample is transformed into nitrogen gas, which is subsequently measured by burning it in an oxygen-rich atmosphere. This standard surpasses conventional techniques like the Kjeldahl method in ensuring excellent accuracy, safety, and efficiency in the detection of nitrogen and protein contents. The use of ISO 16634-2:2016 supports regulatory compliance and quality assurance in the production of food and feed by facilitating consistency and reliability in nutritional analysis.
ISO 16634-2:2016 Test Method
The test method for determining the total nitrogen content and calculating the crude protein content according to ISO 16634-2:2016 involves several key steps, including sample preparation, combustion, and reduction of nitrogen oxides, detection, measurement, and calculation. This technique is used to determine the nitrogen and protein content of different food products and animal feeds because of its great precision, safety, and speed.
| Preparation of the testing setup | The combustion device is installed correctly and according to the manufacturer’s instructions. This involves ensuring that everything is securely fastened and positioned steadily to avoid vibrations and other disruptions while it is operating. |
| Evaluation of the test | Utilize the reduction furnace to transform the nitrogen oxides created during combustion into nitrogen gas (N2). Utilizing the thermal conductivity detector (TCD), determine the nitrogen gas content. Note the nitrogen gas production amount as indicated by the detector readings. |
ISO 16634-2:2016 Test Specimens
| Specimen Detail | According to ISO 16634-2:2016, a test specimen should be a sample indicative of the full batch of food product or animal feed. |
| Specimen Size | The sample size is sufficient for multiple analyses. It is usually advised to use at least 100 grams for homogenization and further testing. |
